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Request |
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NBRP ID |
S90791 |
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Strain Name |
Flamindo2 in gcΔ (sgcAΔ, gcAΔ) |
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Strain Summary |
Flamindo2 as a cAMP probe in gcΔ(sgcAΔ, gcAΔ) |
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Strain Descriptor |
gcΔ(sgcAΔ, gcAΔ) cells expressing Flamindo2 |
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Systematic Name |
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Species |
Dictyostelium discoideum |
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Category |
Recombinant |
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Parental Strain |
gcΔ (S00337) |
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Mutagenesis Method |
extrachromosomal plasmid |
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Mutagenesis Detail |
Flamindo2 was inserted into pHK12(neo) vector |
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Associated Gene |
sgcA
gcA
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Foreign Gene |
Flamindo2, neoR |
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Genotype |
axeA1, axeB1, axeC1, pyr5-6-[pRG24], sgcA-[psCycKOpyr],ura+,gca-[ddgca-bsr], Flamindo2[pHK12::neoR] |
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Phenotype |
development defect, G418 resistant, blasticidin resistant |
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Axenic Growth |
Y |
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Viable Spore Formation |
N |
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References |
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Other Information |
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Depositor |
Hidenori Hashimura+W107 |
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Position |
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Source |
depositor |
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Use Condition |
B1 |
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DSC |
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Remarks |
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Images |
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Sequence |
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